Obtain related molecules according to cell phenotype, and explore drug action mechanism and target (mainly in vitro cell level). According to the development stages of the above NCI screening model, we can see that the cell screening-based model has gradually become an essential part. The main advantages of the cell screening model: low cost, simple operation, and fast results; low drug consumption, especially for natural products with difficult material collection; simultaneous screening of multiple human-derived cell lines may screen effective drugs for special human tumors. What can you get from cell experiments? High-throughput screening of candidate compounds; preliminary understanding of the antitumor spectrum of candidate compounds; providing references for subsequent in vivo experiments, such as dose ranges, tumor types, etc. Common cell experimental technology NCI-60 This screening mode is mainly for drugs with unknown targets. Generally, it is based on phenotypic screening (cell proliferation). If it is found that the drug can inhibit cell growth, metastasis or angiogenesis better The effect can be further combined with animal results to verify the efficacy. Further explore the target of drug action [2]. Such as platinum antitumor drugs, representative drugs: cisplatin and oxaliplatin. First, platinum was found to have a good function of inhibiting the proliferation of multiple tumor cell lines; further in vivo verification has a good anti-cancer effect; and finally It was found that the combination of cisplatin and DNA interfered with normal division and killed cancer cells; furthermore, the position of the combination of platinum and DNA bases was determined by X-ray diffraction technology of NMR broad spectrum analysis of single crystal. As shown in the figure below, the screening process for unknown target drugs: Part 2: Emerging Targeted Drug Screening Models Traditional anticancer drugs, that is, broad-spectrum chemotherapy drugs, in addition to killing cancer cells, will also affect normal cells, such as platinum Class drugs have strong renal toxicity. Therefore, the development of targeted drugs was promoted in the early 21st century.
