Nude mouse subcutaneous tumor-bearing steps are generally more The time is to choose human tumor cell lines, but due to the existence of immune rejection, nude mice need to be tumor-bearing. ⑵ Quantity: each group when tumor-bearing laboratory plastic ware No less than 10, taking into account the tumor formation rate and the cause of death of the mouse, at least 6 valid data should be obtained in the final experiment; ⑶ administration time: generally 7-10 days after vaccination See the tumor growing, you can group experiments (rat cell line will be faster); ⑷ Humane endpoint: animal ethics regulations, mouse tumor weight must not exceed 10% of mouse weight, the average tumor diameter does not exceed 20mm, and if When ulceration occurs, causing infection or necrosis, the experiment should be stopped and the animals euthanized. Why does the tumor fail to grow? On the one hand, it may be that the cell state is poor, the vitality is not good, or the tumor cells are not easy to form tumors. Calculate the cell inoculation volume. ⑶ Poor uniformity, large gap in tumor volume? If the tumor-bearing group finds that there is a large difference in the tumor volume of the mouse, this may be caused by the inconsistency of the mouse's week age and the amount of inoculated cells. This concludes the introduction of the subcutaneous tumor-bearing experiment. Human-derived cell line: For xenotransplantation, immune-deficient mice should be selected (eg nude, NOD / scid, NSG, click on the link 3. Because there are multiple tumor masses in a mouse, they may affect each other.) ) ⑷ Operation steps (please watch the video) ① Select a good place to inject the needle during inoculation, it is recommended to wipe the disinfection position with an alcohol cotton ball before inoculation; ② Go forward and slightly inject the needle before injection. Of course, it is best to combine the literature to determine the cell inoculation amount. On the one hand, it may be that the mice are too old or the mice are immune rejection , It is recommended to replace the more immunodeficient mice (Nod-scid mice, NOG mice> Nude nude mice) ⑵ mouse tumors grow up and disappear? It is very common for tumor masses to shrink first and then become larger. Every movement indicates that it is under the skin, otherwise it may be in the skin or in the muscle; ③
