The measurement temperature is generally 37°C for automatic biochemical analyzers, while semi-automatic biochemical analyzers have room temperature, 25~C, 30cc, 37~C, etc. The endpoint method is performed outside the machine, so choosing room temperature can reduce the time for instrument temperature adjustment. , Improve work efficiency, and it is more appropriate to choose 37℃ for the rate method and the two-point method.2.4 Sample volume fraction The sample volume fraction is the ratio of the sample volume to the total volume of the reaction solution (sv/TV). In the determination of enzyme activity, the proportion of the sample in the total volume should be less than 10%. Generally speaking, if the content of the test substance in the sample is low and the physiological fluctuation range is large, the sample amount is larger. For example, the Trinder reaction measures serum glucose, cholesterol, etc. The ratio of sample reagents is mostly 1:100, and the ratio of blood uric acid or high-density lipoprotein cholesterol is often increased to 1:50; the ratio of AIJT and AST samples is mostly 1:10 to 1 : 20. The method is sensitive and high absorbance experimental method, the sample reagent ratio is small, such as the albumin BCG method, the sample reagent ratio is often 1:200. The Insulin Syringe Assembly Machine method has a short monitoring time and a low absorbance value, and the ratio of sample reagents is mostly 1:10. Generally, the instructions of the reagents should prevail and should not be changed easily. The amount of sample and reagent can be calculated based on the amount of the final reaction solution.
